Overview

  • Product nameAnti-CD45.1 antibody [A20]
    See all CD45.1 primary antibodies
  • Description
    Mouse monoclonal [A20] to CD45.1
  • Tested applicationsSuitable for: IHC-FoFr, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    ab25078 recognizes all mouse leukocytes expressing the CD45.1 allotype and is useful as a marker for origin of donor and host cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17626103
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • RelevanceCD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • LY5 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Receptor type tyrosine protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45.1 antibody [A20] images

  • Flow Cytometry analysis of SJL mouse splenocytes labeling CD45.1 with ab25078 at 0.1 μg/106 cells (Purple). A Goat Anti-Mouse IgG2a, Human ads-AF647 was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-AF647.

References for Anti-CD45.1 antibody [A20] (ab25078)

This product has been referenced in:
  • Soehnlein O  et al. Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes. EMBO Mol Med 5:471-81 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23417922) »
  • Antoine-Moussiaux N  et al. A non-cytosolic protein of Trypanosoma evansi induces CD45-dependent lymphocyte death. PLoS One 4:e5728 (2009). Mouse . Read more (PubMed: 19478957) »

See all 3 Publications for this product

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