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The details of the immunogen for this antibody are not available.
ab25078 recognizes all mouse leukocytes expressing the CD45.1 allotype and is useful as a marker for origin of donor and host cells.
Our Abpromise guarantee covers the use of ab25078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 17626103|
|Flow Cyt||Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Flow Cytometry analysis of SJL mouse splenocytes labeling CD45.1 with ab25078 at 0.1 μg/106 cells (Purple). A Goat Anti-Mouse IgG2a, Human ads-AF647 was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-AF647.