Customer reviews and FAQs

Overview

  • Product nameAnti-CD45.1 antibody [A20] (FITC)
    See all CD45.1 primary antibodies
  • Description
    Mouse monoclonal [A20] to CD45.1 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody recognizes all mouse leukocytes expressing the CD45.1 allotype.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    This antibody is useful as a marker for origin of donor and host cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab24917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • RelevanceCD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
  • Cellular localizationType I membrane protein.
  • Database links
    • Alternative names
      • B220 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • LY5 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Receptor type tyrosine protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    References for Anti-CD45.1 antibody [A20] (FITC) (ab24917)

    This product has been referenced in:
    • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Mouse . Read more (PubMed: 25936350) »
    • Salmond RJ  et al. The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals. Nat Immunol 15:875-83 (2014). Read more (PubMed: 25108421) »

    See all 3 Publications for this product

    Publishing research using ab24917? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab24917 Submit a question for ab24917

    1 Abreview or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45.1 antibody [A20] (FITC)

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Rodent Block M (Biocare Medical) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution pH6.1 (DAKO)
    Sample Mouse Tissue sections (Spleen)
    Specification Spleen
    Permeabilization No
    Fixative Paraformaldehyde
    Read More
    abreview image
    Username

    Mr. Eike Wegener

    Verified customer

    Submitted Sep 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"