Anti-CD45.1 antibody [NDS 58] (FITC) (ab33925)

Overview

  • Product nameAnti-CD45.1 antibody [NDS 58] (FITC)
    See all CD45.1 primary antibodies
  • Description
    Rat monoclonal [NDS 58] to CD45.1 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityMCA415F recognises rat CD45.1 (RT7.1), expressed by rat strains including AO, BN, DA, LEW and PVG. The antibody does not react with the RT7.2 alloantigen.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Thymocytes from LEW rat.

  • Positive control
    • Spleen cells

Properties

Applications

Our Abpromise guarantee covers the use of ab33925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Neat - 1/5.
    Use 10µl of the suggested working dilution to label 106 cells in 100µl.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
    • Cellular localizationType I membrane protein.
    • Database links
    • Alternative names
      • B220 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • LY5 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Receptor type tyrosine protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Anti-CD45.1 antibody [NDS 58] (FITC) images

    • Staining of rat spleen cells with rat anti-rat CD45.1 (FITC) antibody (ab33925).

    References for Anti-CD45.1 antibody [NDS 58] (FITC) (ab33925)

    ab33925 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"