• Product nameAnti-CD45R antibody [YKIX 753.22.2]
    See all CD45R primary antibodies
  • Description
    Rat monoclonal [YKIX 753.22.2] to CD45R
  • Specificityab22514 recognises CD45R on subsets of leucocytes (neutrophils are weak) in canine blood. it may recognise a CD45R isoform.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Dog
  • Immunogen

    Tissue/ cell preparation (Dog): Canine thymocytes



Our Abpromise guarantee covers the use of ab22514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.

Use 10µl for 106 cells.

ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

It precipitates an antigen of 220kD from Con A Blasts.

Functional Studies Use at an assay dependent concentration.

Complement lysis: Use at an assay dependent dilution.


  • FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • DomainThe first PTPase domain interacts with SKAP1.
  • Post-translational
    Heavily N- and O-glycosylated.
  • Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • Glycoprotein antibody
    • GP180 antibody
    • L CA antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • Leukocyte common antigen precursor antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor type tyrosine protein phosphatase C antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

References for Anti-CD45R antibody [YKIX 753.22.2] (ab22514)

This product has been referenced in:
  • Alves EG  et al. Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs. BMC Vet Res 10:190 (2014). Flow Cyt ; Dog . Read more (PubMed: 25178540) »
  • Cobbold S & Metcalfe S Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens 43:137-54 (1994). Read more (PubMed: 8091414) »

See all 2 Publications for this product

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