Anti-CD45RA antibody [4KB5] (ab755)

Overview

  • Product name
    Anti-CD45RA antibody [4KB5]
    See all CD45RA primary antibodies
  • Description
    Mouse monoclonal [4KB5] to CD45RA
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human hairy cell leukaemia cells.

  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notes
    Immunohistochemistry: 1 / 10 - 1 / 25 for 30 - 60 mins (ABC method).
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    This antibody is not suitable for Zenker's fixed tissue sections.
  • Target

    • Function
      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
    • Involvement in disease
      Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
      Multiple sclerosis
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.
    • Domain
      The first PTPase domain interacts with SKAP1.
    • Post-translational
      modifications
      Heavily N- and O-glycosylated.
    • Cellular localization
      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • B220 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • Loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • Protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Images

    • ab755 staining human tonsil by IHC-P.

    • ab755 staining CD45RA in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.

    References

    ab755 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    The concentration of the current lot of ab8260 that we have in stock is 2.0 mg/ml. However, they also informed me that this concentration does vary from lot to lot. Additionally, they have also let me know that the way the concentration was determined ...

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    Thank you for your enquiry. Ab755 has been tested for application in Immunohistochemistry, both formalin-fixed paraffin-embedded sections and frozen sections. It is recommended to use this antibody at a dilution of 1:10 - 1:25 in an ABC method and an i...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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