Anti-CD45RA antibody [HI100], prediluted (PerCP/Cy5.5®) (ab155362)

Overview

  • Product name
    Anti-CD45RA antibody [HI100], prediluted (PerCP/Cy5.5®)
    See all CD45RA primary antibodies
  • Description
    Mouse monoclonal [HI100] to CD45RA, prediluted (PerCP/Cy5.5®)
  • Host species
    Mouse
  • Conjugation
    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Normal Human peripheral blood cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

Target

  • Function
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • Involvement in disease
    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Domain
    The first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications
    Heavily N- and O-glycosylated.
  • Cellular localization
    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • Loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase, receptor type C antibody
    • Protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Images

  • Flow cytometric analysis of normal Human peripheral blood cells with mouse IgG2b kappa Isotype Control PerCP-Cy5.5 (open histogram) or f ab155362 PerCP-Cy5.5 (filled histogram). Cells in the lymphocyte gate were used for analysis.

References

ab155362 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Permeabilization
No
Sample
Human Cell (PBMC)
Specification
PBMC
Gating Strategy
lymphocytes
Preparation
Cell harvesting/tissue preparation method: Ficoll
Sample buffer: DPBS
Username

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Verified customer

Submitted Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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