The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive Multiple sclerosis
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains.
The first PTPase domain interacts with SKAP1.
Heavily N- and O-glycosylated.
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
Flow cytometric analysis of normal Human peripheral blood cells with mouse IgG2b kappa Isotype Control PerCP-Cy5.5 (open histogram) or f ab155362 PerCP-Cy5.5 (filled histogram). Cells in the lymphocyte gate were used for analysis.