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Tissue/ cell preparation (Human). (Thymocytes and T lymphocytes).
Our Abpromise guarantee covers the use of ab8217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.|
|WB||Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.|
|Flow Cyt||Use a concentration of 1 - 4 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|