The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: This reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul whole blood.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive Multiple sclerosis
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains.
The first PTPase domain interacts with SKAP1.
Heavily N- and O-glycosylated.
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
Cortez-Retamozo V et al. Origins of tumor-associated macrophages and neutrophils. Proc Natl Acad Sci U S A109:2491-6 (2012).
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Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha)35:289-97 (1989).
Read more (PubMed: 2534480) »