Anti-CD45RA antibody [MEM-56], prediluted (Allophycocyanin) (ab30507)

Submit an Abreview Submit a Question Specific References (3)

Overview

  • Product name
    Anti-CD45RA antibody [MEM-56], prediluted (Allophycocyanin)
    See all CD45RA primary antibodies
  • Description
    Mouse monoclonal [MEM-56] to CD45RA, prediluted (Allophycocyanin)
  • Host species
    Mouse
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity
    ab30507 recognises CD45RA antigen.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Thymocytes and T lymphocytes (Human)

  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Properties

Applications

Our Abpromise guarantee covers the use of ab30507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20µl for 106 cells or 100 ul of whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
    • Involvement in disease
      Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
      Multiple sclerosis
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.
    • Domain
      The first PTPase domain interacts with SKAP1.
    • Post-translational
      modifications
      Heavily N- and O-glycosylated.
    • Cellular localization
      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
      • Alternative names
        • B220 antibody
        • CD45 antibody
        • CD45 antigen antibody
        • CD45R antibody
        • EC 3.1.3.48 antibody
        • GP180 antibody
        • L-CA antibody
        • LCA antibody
        • Leukocyte common antigen antibody
        • Loc antibody
        • Ly-5 antibody
        • LY5 antibody
        • Ly5, homolog of antibody
        • Lymphocyte antigen 5 antibody
        • Lyt-4 antibody
        • Protein tyrosine phosphatase, receptor type C antibody
        • Protein tyrosine phosphatase, receptor type, C antibody
        • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
        • Ptprc antibody
        • PTPRC_HUMAN antibody
        • Receptor-type tyrosine-protein phosphatase C antibody
        • RT7 antibody
        • T200 antibody
        • T200 glycoprotein antibody
        • T200 leukocyte common antigen antibody
        see all

      Images

      • Flow Cytometry - Anti-CD45RA antibody [MEM-56], prediluted (Allophycocyanin) (ab30507)
        Flow Cytometry - Anti-CD45RA antibody [MEM-56], prediluted (Allophycocyanin) (ab30507)

        Surface staining of human peripheral blood with anti-CD45RA (MEM-56) APC. 

      References

      This product has been referenced in:
      • Simmons R  et al. High frequency, sustained T cell responses to PARV4 suggest viral persistence in vivo. J Infect Dis 203:1378-87 (2011). Flow Cyt ; Human . Read more (PubMed: 21502079) »
      • Di Mitri D  et al. Reversible senescence in human CD4+CD45RA+CD27- memory T cells. J Immunol 187:2093-100 (2011). Flow Cyt ; Human . Read more (PubMed: 21788446) »

      See all 3 Publications for this product

      Publishing research using ab30507? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab30507.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up