Anti-CD45RA antibody [MEM-56], prediluted (Allophycocyanin) (ab30507)

Overview

• Product name
  Anti-CD45RA antibody [MEM-56], prediluted (Allophycocyanin)
  See all CD45RA primary antibodies
• Description
  Mouse monoclonal [MEM-56] to CD45RA, prediluted (Allophycocyanin)
• Conjugation
  Allophycocyanin. Ex: 645nm, Em: 660nm
• Specificity
  ab30507 recognises CD45RA antigen.
• Tested applications
  Suitable for: Flow Cytmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Thymocytes and T lymphocytes (Human)

• General notes

  The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Properties

• Form
  Prediluted
• Storage instructions
  Shipped at 4°C. Store at +4°C.
• Storage buffer
  Preservative: 15mM Sodium Azide
  Constituents: 0.2% BSA, PBS
• Concentration information loading...
• Purity
  IgG fraction
• Clonality
  Monoclonal
• Clone number
  MEM-56
• Isotype
  IgG2b
• Research areas
  • Immunology
  • Adaptive Immunity
  • B Cells
  • CD

  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

  • Immunology
  • Cell Type Markers
  • CD
  • Non-lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • B Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Target

• Function
  Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
• Involvement in disease
  Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
  Multiple sclerosis
• Sequence similarities
  Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
  Contains 2 fibronectin type-III domains.
  Contains 2 tyrosine-protein phosphatase domains.
• Domain
  The first PTPase domain interacts with SKAP1.
• Post-translational
  modifications
  Heavily N- and O-glycosylated.
• Cellular localization
  Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.

• Target information above from: UniProt accession P08575 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 5788 Human
  • Omim: 151460 Human
  • SwissProt: P08575 Human
  • Unigene: 654514 Human
• Alternative names
  • B220 antibody
  • CD45 antibody
  • CD45 antigen antibody

  • CD45R antibody
  • EC 3.1.3.48 antibody
  • GP180 antibody
  • L-CA antibody
  • LCA antibody
  • Leukocyte common antigen antibody
  • Loc antibody
  • Ly-5 antibody
  • LY5 antibody
  • Ly5, homolog of antibody
  • Lymphocyte antigen 5 antibody
  • Lyt-4 antibody
  • Protein tyrosine phosphatase, receptor type C antibody
  • Protein tyrosine phosphatase, receptor type, C antibody
  • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
  • Ptprc antibody
  • PTPRC_HUMAN antibody
  • Receptor-type tyrosine-protein phosphatase C antibody
  • RT7 antibody
  • T200 antibody
  • T200 glycoprotein antibody
  • T200 leukocyte common antigen antibody

  see all

Protocols

• Flow cytometry protocols