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Purified spleen leucocyte common antigen.
Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line. This clone has been described reacting with paraffin embedded material following PLP fixation (see Whiteland et al., 1995). It only labels B cells among thoracic duct lymphocytes, with little labelling in bone marrow and none on thymocytes (Barclay et al., 1987).
Our Abpromise guarantee covers the use of ab33933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"