Anti-CD45RC antibody [C455.1F] (Phycoerythrin) (ab25521)

Overview

  • Product nameAnti-CD45RC antibody [C455.1F] (Phycoerythrin)
    See all CD45RC primary antibodies
  • Description
    Rat monoclonal [C455.1F] to CD45RC (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody is specific to CD45RC.
  • Tested applicationsSuitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • EpitopeThis antibody reacts with an exon C dependent epitope of CD45 glycoprotein.
  • Positive control
    • Cells from BALB/c spleen for Immunofluorescent staining with Flow cytometric analysis.

Properties

Applications

Our Abpromise guarantee covers the use of ab25521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IP Use at an assay dependent dilution.

Target

  • RelevanceThe CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
  • Cellular localizationMembrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
  • Database links
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • PTPRC antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 Glycoprotein antibody
    see all

Anti-CD45RC antibody [C455.1F] (Phycoerythrin) images

  • Ab25521 (at a concentration of 0.2µg for 106 cells) and rat anti mouse CD8a FITC doubly staining CD45RC in cells from BALB/c spleen. After staining, small lymphocytes were gated and analyzed on a flow cytometer.

References for Anti-CD45RC antibody [C455.1F] (Phycoerythrin) (ab25521)

ab25521 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"