Overview

  • Product name
    Anti-CD45RC antibody [IBL-8]
    See all CD45RC primary antibodies
  • Description
    Rat monoclonal [IBL-8] to CD45RC
  • Host species
    Rat
  • Specificity
    This antibody recognises the exon C-dependent RC isoform of CD45. It reacts strongly with B cells and less intensely with most CD8+ T cells. It does not recognise CD4+ T cells.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance
    The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
  • Cellular localization
    Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
  • Database links
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • PTPRC antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 Glycoprotein antibody
    see all

Images

  • Immunohistochemistry of mouse spleen staining CD45RC using Anti-CD45RC antibody [IBL-8] (ab3135)

     

References

This product has been referenced in:
  • Zhao HY  et al. Prognostic significance of thymidylate synthase in postoperative non-small cell lung cancer patients. Onco Targets Ther 7:1301-10 (2014). IHC . Read more (PubMed: 25114572) »

See 1 Publication for this product

Customer reviews and Q&As

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I am sorry for the delay in my answer. I was waiting for an answer from the sources of these antibodies.

I can confirm that both antibodies were not tested from the lab with other CD45 isoforms.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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