Anti-CD45RC antibody [MRC OX-22] (ab33945)


  • Product nameAnti-CD45RC antibody [MRC OX-22]
    See all CD45RC primary antibodies
  • Description
    Mouse monoclonal [MRC OX-22] to CD45RC
  • Specificityab33945 recognises rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    PHA stimulated rat lymphocytes.



Our Abpromise guarantee covers the use of ab33945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: 1/100.
    IHC-P: 1/100 - 1/1000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    IHC-Fr: 1/100 - 1/1000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
    • Cellular localizationMembrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
    • Database links
    • Alternative names
      • B220 antibody
      • CD45 antibody
      • CD45R antibody
      • EC antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • PTPRC antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 Glycoprotein antibody
      see all

    References for Anti-CD45RC antibody [MRC OX-22] (ab33945)

    ab33945 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Spleen, Lung)
    Specification Spleen, Lung
    Fixative 10% NBF, 24 hours
    Antigen retrieval step Enzymatic

    Abcam user community

    Verified customer

    Submitted Jul 11 2012

    Thank you for your recent telephone enquiry. I have obtained the following general testing method from the originator of ab33933 and ab33934. Please note that this protocol is a guideline only and may need to be optimized in your laboraotry. I h...

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