Anti-CD45RC antibody [MRC OX-22] (Biotin) (ab33937)

Overview

  • Product name
    Anti-CD45RC antibody [MRC OX-22] (Biotin)
    See all CD45RC primary antibodies
  • Description
    Mouse monoclonal [MRC OX-22] to CD45RC (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    PHA stimulated rat lymphocytes

  • Positive control
    • rat splenocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab33937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use neat. Use 10µl of the suggested working dilution to label 106 cells in 100µl.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
    • Cellular localization
      Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
    • Database links
    • Alternative names
      • B220 antibody
      • CD45 antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • PTPRC antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 Glycoprotein antibody
      see all

    Images

    • Flow Cytometry analysis of rat splenocytes labeling CD45RC with ab33937.

    References

    ab33937 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Our labs follow the suggested protocols posted within our Protocol and Troubleshooting Page athttp://www.abcam.com/index.html?pageconfig=popular_protocols. The indirect flow cytometry protocol may be accessed dir...

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    Optimal dilution for AB33937 from our QC testing is 1:2. We used 10ul of this working dilution to stain one million cells in 100ul total volume (an additional 1:10 dilution). For the streptavidin detection reagen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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