Overview

  • Product nameAnti-CD45RO antibody [UCHL-1]
    See all CD45RO primary antibodies
  • Description
    Mouse monoclonal [UCHL-1] to CD45RO
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
    Does not react with: Pig
  • Immunogen

    Antigen isolated from the Il-2 dependent T cell line CA-1.

  • Positive control
    • Tonsil, Thymus
  • General notes


    ab86080 can be used in lymphoma diagnosis and is clustered as CD45RO.

Properties

Applications

Our Abpromise guarantee covers the use of ab86080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 185 kDa.
IHC-P 1/5 - 1/10. Antigen retrieval is not essential but may optimise staining.

Suggested dilutions provided are based on the indirect method, but with an ABC method the dilution can be 5-10 times higher. The optimal dilution should, however, be tested by serial dilution. Dilute immediately before with PBS or Tris HCl pH 7.4.

IHC-Fr 1/5 - 1/10.

Suggested dilutions provided are based on the indirect method, but with an ABC method the dilution can be 5-10 times higher. The optimal dilution should, however, be tested by serial dilution. Dilute immediately before with PBS or Tris HCl pH 7.4.

Target

  • FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • DomainThe first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications
    Heavily N- and O-glycosylated.
  • Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • cd45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • lca antibody
    • Leukocyte common antigen antibody
    • LY 5 antibody
    • LY5 antibody
    • Protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

References for Anti-CD45RO antibody [UCHL-1] (ab86080)

This product has been referenced in:
  • Smith SH  et al. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology 58:63-70 (1986). Read more (PubMed: 2423439) »

See 1 Publication for this product

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Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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