SpecificityRecognizes the low molecular weight (Mr 180kDa) isoform of the leucocyte common antigen (LCA) which is part of a family of protein tryosine phosphatases. The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.
Concentration of the product was found at OD280nm.
NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.
When activated with phytohemagglutinin (PHA), naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. Therefore, Anti-CD45RO may be a marker for memory T-cells. Anti-CD45RO may be used in formalin-fixed and paraffin-embedded tissues as well as frozen sections and cell suspensions.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
Post-translational modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.