Overview

  • Product nameAnti-CD46 antibody [JM6C11]
    See all CD46 primary antibodies
  • Description
    Mouse monoclonal [JM6C11] to CD46
  • SpecificityThis antibody is specific for the pig homologue of human CD46, a 45-65kD glycoprotein also known as Membrane Cofactor Protein (MCP).
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    Pig peripheral blood mononuclear cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberJM6C11
  • MyelomaSp2/0-Ag14
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFACS: 1/10.
    Use 10 ul of 1/10 dilution to label 106 cells in 100ul.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
    • Tissue specificityExpressed by all cells except erythrocytes.
    • Involvement in diseaseDefects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations.
    • Sequence similaritiesContains 4 Sushi (CCP/SCR) domains.
    • DomainSushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.
    • Post-translational
      modifications
      N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
      Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
      In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck.
    • Cellular localizationCytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
    • Information by UniProt
    • Alternative names
      • AHUS2 antibody
      • Antigen defined by monoclonal antibody TRA 2 10 antibody
      • Antigen identified by monoclonal antibody TRA 2 10 antibody
      • CD46 antibody
      • CD46 antigen antibody
      • CD46 antigen complement regulatory protein antibody
      • CD46 molecule antibody
      • CD46 molecule complement regulatory protein antibody
      • Complement membrane cofactor protein antibody
      • MCP antibody
      • MCP_HUMAN antibody
      • Measles virus receptor antibody
      • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody
      • Membrane cofactor protein antibody
      • MGC26544 antibody
      • MIC10 antibody
      • TLX antibody
      • TRA2.10 antibody
      • Trophoblast leucocyte common antigen antibody
      • Trophoblast leukocyte common antigen antibody
      • Trophoblast lymphocyte cross reactive antigen antibody
      see all

    Anti-CD46 antibody [JM6C11] images

    • ab34132 at a 1/10 staining pig peripheral blood lymphocytes using FACS.

      10 ul of 1/10 dilution to label 106 cells in 100ul.

    References for Anti-CD46 antibody [JM6C11] (ab34132)

    ab34132 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"