- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CD47 antibody
See all CD47 primary antibodies -
DescriptionRabbit polyclonal to CD47
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Other Immunogen Type corresponding to Human CD47 aa 20-140.
Sequence:LLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFD GALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTEL TREGETIIELKYRVVSWFSPN
Database link: Q08722 -
Positive control
- WB: MCF-7 and SK-OV-3 cell lysates; Mouse lung lysate. ICC/IFF: MCF-7 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab175388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/500 - 1/2000. Predicted molecular weight: 35 kDa. |
Target
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FunctionHas a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
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Tissue specificityVery broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
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Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 961 Human
- Entrez Gene: 16423 Mouse
- Omim: 601028 Human
- SwissProt: Q08722 Human
- SwissProt: Q61735 Mouse
- Unigene: 446414 Human
- Unigene: 31752 Mouse
- Unigene: 390865 Mouse
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Alternative names
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
see all
Images
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Western blot - Anti-CD47 antibody (ab175388)All lanes : Anti-CD47 antibody (ab175388) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : SK-OV-3 (human ovary adenocarcinoma cell line) cell lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDaBlocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 90 seconds.
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Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody (ab175388)MCF-7 (human breast adenocarcinoma cell line) cells satined for CD47 (green) using ab175388 in ICC/IF.
DAPI nuclear staining in blue.
Protocols
References
ab175388 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"