Overview

  • Product name
  • Description
    Goat polyclonal to CD47
  • Host species
    Goat
  • Specificity
    ab193940 is expected to recognize both reported isoforms (NP_001768.1; NP_942088.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CD47 aa 294-304 (internal sequence) (Cysteine residue). NP_001768.1; NP_942088.1.
    Sequence:

    SNQKTIQPPRK


    Database link: Q08722

  • Positive control
    • Human hippocampus, HeLa and Jurkat lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab193940 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50, 60 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
  • Tissue specificity
    Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody 1D8 antibody
    • Antigenic surface determinant protein OA3 antibody
    • CD 47 antibody
    • CD47 antibody
    • CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
    • CD47 antigen antibody
    • CD47 glycoprotein antibody
    • CD47 molecule antibody
    • CD47_HUMAN antibody
    • IAP antibody
    • Integrin Associated Protein antibody
    • Integrin associated signal transducer antibody
    • Integrin-associated protein antibody
    • Leukocyte surface antigen CD47 antibody
    • MER 6 antibody
    • MER6 antibody
    • OA 3 antibody
    • OA3 antibody
    • OTTHUMP00000041152 antibody
    • OTTHUMP00000041153 antibody
    • Protein MER6 antibody
    • Rh related antigen antibody
    • Surface antigen identified by monoclonal antibody 1D8 antibody
    see all

Images

  • All lanes : Anti-CD47 antibody (ab193940) at 0.3 µg/ml

    Lane 1 : Human hippocampus lysate (in RIPA buffer)
    Lane 2 : HeLa lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 35 kDa


    Exposure time: 1 hour


    The observed molecular weights correspond to glycosylated forms.

References

ab193940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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