Anti-CD47 antibody [B6H12.2] (ab3283)

dear Sir,
I ordered to your company the following antibody: anti-CD47 ab3283
I have a question concerning the epitope; is it well in the extracellular part of CD47?
I thank you very much in advance
sincerely yours

ANSWER:

Thank you for contacting us.

This antibody recognizes the Ig domain. According to the information provided by Uniprot for Q08722 the Ig domain is located within aa 19-127, which is in the extracellular domain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I am interested in some of your products. I wonder if the Anti-CD47 antibody[B6H12.2](ab3283) and Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328) crossreact with cynomolgus monkeys. If they crossreact with monkeys, could you recommend any antibody which is specific for human but doesn't crossreact with cynomolgus monkeys? Thank you very much!

ANSWER:

Thank you for contacting Abcam.

The products ab3283 and ab70328 have not been tested for cross reactivity withcynomolgus monkeys. I was unable to find the associated cynomolgis proteins to the human proteins that many of the CD47 and HLAC1 antibodies are created against. However, if you are able to find the proteins sequences I would gladly do an alignment. Should you wish to perform the alignment for yourself, when homology is less than 85% we usually believe that there is minimal cross reactivity.

I hope this information is helpful. Please let me know if you have any questions.

We are planning to do some CD47-measurements with to different antibody-clones, that mustn't compete for the same CD47-binding site, but both habe to bind the protein at the same time. Up to now we couldn't find any information about the epitope specifity of commerzialized anti-CD47 antibodies. We'd be very pleased if you could possibly provide some further information concerning this issue.

ANSWER:

Thank you for your enquiry. For ab3283, the information that we regarding the epitope is that it is the Ig domain. For ab9089, the epitope has not yet been mapped. If you have any more questions, please contact us again.

We are interested in working with your product CD47 antibody [B6H12.2] or CD47 antibody [MEM-122]. According to your data sheets and the literature they work well with primate cells. Since we use mouse cells, however, we would like to know whether you have any additional data about the specificity of the antibodies. If not, would it be possible for you to provide us a small aliqout for testing the reactivity on material derived from mice?

ANSWER:

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement.

Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.