For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Intact CD47 purified from placenta.
Our Abpromise guarantee covers the use of ab3283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|Functional Studies||Use at an assay dependent dilution. This antibody blocks the binding of SIRP alpha (Yoshida, et al.; Latour et al.) and inhibits in assays where CD47-integrin association is required.|
|ICC||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution. (native, with protein G): use at a concentration of 2µg / mg protein lysate.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 20923773|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22451919|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 35 kDa).|
Overlay histogram showing peripheral blood lymphocytes stained with ab3283 (red line). The cells were incubated with the antibody (ab3283, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"