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Intact CD47 purified from placenta.
Our Abpromise guarantee covers the use of ab3283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|Functional Studies||Use at an assay dependent concentration. This antibody blocks the binding of SIRP alpha (Yoshida, et al.; Latour et al.) and inhibits in assays where CD47-integrin association is required.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. (native, with protein G): use at a concentration of 2µg / mg protein lysate.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20923773|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22451919|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 35 kDa).|
Overlay histogram showing peripheral blood lymphocytes stained with ab3283 (red line). The cells were incubated with the antibody (ab3283, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
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