Overview

  • Product nameAnti-CD47 antibody [EPR4150(2)]See all CD47 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR4150(2)] to CD47
  • Tested applicationsWB, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the intracellular domain of Human CD47.

  • Positive control
    • Jurkat, HeLa, and K562 cell lysates; Jurkat cells
  • General notesProduced under U.S. Patent No. 5,675,063.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab108415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 35 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionHas a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
    • Tissue specificityVery broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
    • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody 1D8 antibody
      • Antigenic surface determinant protein OA3 antibody
      • CD 47 antibody
      • CD47 antibody
      • CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
      • CD47 antigen antibody
      • CD47 glycoprotein antibody
      • CD47 molecule antibody
      • CD47_HUMAN antibody
      • IAP antibody
      • Integrin Associated Protein antibody
      • Integrin associated signal transducer antibody
      • Integrin-associated protein antibody
      • Leukocyte surface antigen CD47 antibody
      • MER 6 antibody
      • MER6 antibody
      • OA 3 antibody
      • OA3 antibody
      • OTTHUMP00000041152 antibody
      • OTTHUMP00000041153 antibody
      • Protein MER6 antibody
      • Rh related antigen antibody
      see all

    Anti-CD47 antibody [EPR4150(2)] images

    • All lanes : Anti-CD47 antibody [EPR4150(2)] (ab108415) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
    • Flow cytometric analysis of CD47 in permeabilized Jurkat cells using ab108415 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References for Anti-CD47 antibody [EPR4150(2)] (ab108415)

    This product has been referenced in:
    • Malek MH  et al. Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks of endurance exercise in mice: discontinuous versus continuous training. Exp Physiol 98:807-18 (2013). Read more (PubMed: 23180811) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    Apologies as there was an error in writing the catalogue number. It should be ab33852.

    Ab108415 has tested as non reactive in IHC-P so the Abtrial programme is not valid.

    I hope this information i...

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    Thank you for contacting us.

    Unfortunately we do not have antibody that is tested in IHC-P with Rat sections. You may recommend ab33582 with Abtrial promotion.

    I hope this information is helpful to you. Please do not hesitate to conta...

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    Thank you for contacting us.

    Unfortunately the product is unsuitable for IHC-P. I am sorry the IHC-P application is not recommended for this antibody so Abtrial discount cannot be provided.

    I hope this information is helpful to you. P...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"