Overview

  • Product nameAnti-CD47 [OX-101] antibodySee all CD47 primary antibodies ...
  • Description
    Mouse monoclonal [OX-101] to CD47
  • SpecificityAb33852 recognises CD47 expressed primarily by lymphocytes and macrophages but also detected at high levels on brain endothelial cells.
  • Tested applicationsFlow Cyt, IP, WB, Functional Studies more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation: Rat thymocytes

  • General notes


    OX-101 (this clone) recognises the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesAb33852 was purified from tissue culture supernatant.
  • Primary antibody notes OX-101 (this clone) recognises the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).
  • Clonality Monoclonal
  • Clone numberOX-101
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab33852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
IP
WB
Functional Studies
  • Application notesFlow Cyt: Use 10µl for 106 cells in 100µl.
    FuncS: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 33 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
    • Tissue specificityVery broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
    • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localizationCell membrane.
    • Target information above from: UniProt accession Q08722 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Antigenic surface determinant protein OA3 antibody
      • CD 47 antibody
      • CD47 antibody
      • Cd47 antibody
      • CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
      • CD47 antigen antibody
      • CD47 glycoprotein antibody
      • CD47 molecule antibody
      • CD47_HUMAN antibody
      • IAP antibody
      • Integrin Associated Protein antibody
      • Integrin associated signal transducer antibody
      • Integrin-associated protein antibody
      • Leukocyte surface antigen CD47 antibody
      • MER 6 antibody
      • MER6 antibody
      • OA 3 antibody
      • OA3 antibody
      • OTTHUMP00000041152 antibody
      • OTTHUMP00000041153 antibody
      • Protein MER6 antibody
      • Rh related antigen antibody
      see all

    References for Anti-CD47 [OX-101] antibody (ab33852)

    This product has been referenced in:
    • Vernon-Wilson EF  et al. CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1. Eur J Immunol 30:2130-7 (2000). Read more (PubMed: 10940903) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"