The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/Neat. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Note: Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.