The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesThis antibody may be diluted to a titer of 1:50-1:100 in an ABC method.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
Heat mediated antigen retrieval of formalin fixed paraffin embedded tissues may be required before immunostaining.
FunctionMay act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.