The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25µg for 105 cells. Use at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
ab154460-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
Flow cytometry staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.125 µg of Rat IgG2a ? Isotype Control PE-Cy7 (left) or 0.125 µg of ab95557 (right). Cells in the lymphocyte gate were used for analysis.