Overview

  • Product name
    Anti-CD5 antibody [CD5/54/F6]
    See all CD5 primary antibodies
  • Description
    Mouse monoclonal [CD5/54/F6] to CD5
  • Host species
    Mouse
  • Specificity
    ab193378 is very useful in differentiating among mature small lymphoid cell malignancies. It can also be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD5 (intracellular).
    Database link: P06127

  • Positive control
    • 293T, Ramos, or MOLT4 cells; Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab193378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at 1-2 µg/mg of lysate.

Target

  • Function
    May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
  • Sequence similarities
    Contains 3 SRCR domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 5 antibody
    • CD5 antibody
    • CD5 antigen (p56 62) antibody
    • CD5 antigen antibody
    • CD5 molecule antibody
    • CD5_HUMAN antibody
    • LEU1 antibody
    • LyA antibody
    • Lymphocyte antigen T1/Leu-1 antibody
    • OTTHUMP00000236973 antibody
    • T-cell surface glycoprotein CD5 antibody
    • T1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD5 with ab193378 at 1 µg/ml.

References

ab193378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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