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The details of the immunogen for this antibody are not available.
ab24891 is useful as a pan T cell marker.
Our Abpromise guarantee covers the use of ab24891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with ab24891 and a PE conjugated mouse anti-feline CD8 antibody (ab25417). Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM flow cytometry (BDIS, San Jose, CA).