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The details of the immunogen for this antibody are not available.
This antibody has been shown to be useful as a pan T cell marker.
Our Abpromise guarantee covers the use of ab25415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration.
|IP||Use at an assay dependent concentration.|
Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with a FITC conjugated mouse anti-feline CD4 antibody (ab24894) and ab25415 at 0.2 µg/106 cells. Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScanTM (BDIS, San Jose, CA).
ab25415 has not yet been referenced specifically in any publications.