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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab61020 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 55 kDa).
Function May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
Sequence similarities Contains 3 SRCR domains.
Cellular localization Membrane.
Information by UniProt
CD 5 antibody
CD5 antigen (p56 62) antibody
Anti-CD5 (phospho Y453) antibody images
Western blot - CD5 (phospho Y453) antibody (ab61020)
All lanes : Anti-CD5 (phospho Y453) antibody (ab61020) Lane 1 : 293 cells treated
with PMA (125ng/ml, 30mins) Lane 2 : 293 cells treated
with PMA (125ng/ml, 30mins) with Immunizing phospho-peptide Predicted band size : 55 kDa Observed band size : 42 kDa ( why is the actual band size different from the predicted?)
References for Anti-CD5 (phospho Y453) antibody (ab61020)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"