Overview

  • Product nameAnti-CD5 (phospho Y453) antibody
    See all CD5 primary antibodies
  • Description
    Rabbit polyclonal to CD5 (phospho Y453)
  • Specificityab61020 detects endogenous levels of CD5 only when phosphorylated at tyrosine 453.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from human CD5 around the phosphorylation site of tyrosine 453(N-E-YP-S-Q).

  • Positive control
    • Extracts from 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 55 kDa).
ELISA 1/20000.

Target

Anti-CD5 (phospho Y453) antibody images

  • All lanes : Anti-CD5 (phospho Y453) antibody (ab61020)

    Lane 1 : 293 cells treated with PMA (125ng/ml, 30mins)
    Lane 2 : 293 cells treated with PMA (125ng/ml, 30mins) with Immunizing phospho-peptide


    Predicted band size : 55 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-CD5 (phospho Y453) antibody (ab61020)

ab61020 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"