The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Note: samples should be acetone fixed.
IP: Use at an assay dependent dilution.
Neut: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceIntegrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.
CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets.
Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.
Cellular localizationCell Membrane; single-pass type I membrane protein.
References for Anti-CD51 + CD61 antibody [23C6] (ab20143)
This product has been referenced in:
Martino MM et al. Controlling integrin specificity and stem cell differentiation in 2D and 3D environments through regulation of fibronectin domain stability. Biomaterials30:1089-97 (2009).
Read more (PubMed: 19027948) »
Scarlett A et al. Thyroid hormone stimulation of extracellular signal-regulated kinase and cell proliferation in human osteoblast-like cells is initiated at integrin alphaVbeta3. J Endocrinol196:509-17 (2008).
Read more (PubMed: 18310446) »