Overview

  • Product nameAnti-CD51 + CD61 antibody [23C6]
    See all CD51 + CD61 primary antibodies
  • Description
    Mouse monoclonal [23C6] to CD51 + CD61
  • SpecificityThis antibody recognises the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. The antibody reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Chicken, Human
    Does not react with: Rabbit, Cow, Pig
  • Immunogen

    Osteoclasts from osteoclastomas

Properties

Applications

Our Abpromise guarantee covers the use of ab34226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/40 - 1/80. Use 10µl of suggested dilution for 106 cells in 100µl.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • RelevanceIntegrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.
    • Cellular localizationCell Membrane; single-pass type I membrane protein.
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody L230 antibody
      • CD51 antibody
      • CD51 antigen antibody
      • CD61 antibody
      • CD61 antigen antibody
      • DKFZp686A08142 antibody
      • GP3A antibody
      • GPIIIa antibody
      • Integrin alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • Integrin alpha v antibody
      • Integrin beta 3 (platelet glycoprotein IIIa, antigen CD61) antibody
      • Integrin beta 3 antibody
      • Integrin beta chain beta 3 antibody
      • ITGAV antibody
      • ITGB3 antibody
      • MSK8 antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet membrane glycoprotein IIIa antibody
      • Vitronectin receptor subunit alpha antibody
      • VNRA antibody
      see all

    References for Anti-CD51 + CD61 antibody [23C6] (ab34226)

    This product has been referenced in:
    • Behjati S  et al. Distinct H3F3A and H3F3B driver mutations define chondroblastoma and giant cell tumor of bone. Nat Genet 45:1479-82 (2013). Human . Read more (PubMed: 24162739) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"