• Product nameAnti-CD52 antibody [HI186]
    See all CD52 primary antibodies
  • Description
    Mouse monoclonal [HI186] to CD52
  • SpecificityCD52 antigen
  • Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human tonsil).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by protein G-affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberHI186
  • Myelomaunknown
  • IsotypeIgG2b
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab2576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.


  • FunctionMay play a role in carrying and orienting carbohydrate, as well as having a more specific role.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cambridge pathology 1 antigen antibody
    • CAMPATH 1 antibody
    • CAMPATH 1 antigen antibody
    • CAMPATH-1 antigen antibody
    • CD 52 antibody
    • CD52 antibody
    • CD52 antigen antibody
    • CD52 molecule antibody
    • CD52_HUMAN antibody
    • CDw52 antibody
    • CDW52 antigen antibody
    • Epididymal secretory protein E5 antibody
    • Epididymis secretory sperm binding protein Li 171mP antibody
    • HE 5 antibody
    • He5 antibody
    • Human epididymis-specific protein 5 antibody
    see all

Anti-CD52 antibody [HI186] images

  • Overlay histogram showing Jurkat cells stained with ab2576 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-CD52 antibody [HI186] (ab2576)

This product has been referenced in:

See 1 Publication for this product

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It has been confirmed by HLDA Workshop VI (the best proof of defined specificity). If you have any additional questions, please contact us again.

Immunogen was a human tonsil=tissue in suspension.