Anti-CD52 antibody [HI186], prediluted (FITC) (ab69774)

Overview

  • Product name
    Anti-CD52 antibody [HI186], prediluted (FITC)
    See all CD52 primary antibodies
  • Description
    Mouse monoclonal [HI186] to CD52, prediluted (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human tonsil.

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Size exclusion
  • Clonality
    Monoclonal
  • Clone number
    HI186
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells. ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cambridge pathology 1 antigen antibody
    • CAMPATH 1 antibody
    • CAMPATH 1 antigen antibody
    • CAMPATH-1 antigen antibody
    • CD 52 antibody
    • CD52 antibody
    • CD52 antigen antibody
    • CD52 molecule antibody
    • CD52_HUMAN antibody
    • CDw52 antibody
    • CDW52 antigen antibody
    • Epididymal secretory protein E5 antibody
    • Epididymis secretory sperm binding protein Li 171mP antibody
    • HE 5 antibody
    • He5 antibody
    • Human epididymis-specific protein 5 antibody
    see all

Anti-CD52 antibody [HI186], prediluted (FITC) images

  • Overlay histogram showing peripheral blood lymphocytes stained with ab69774 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69774, 0.06 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b FITC (0.6 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

References for Anti-CD52 antibody [HI186], prediluted (FITC) (ab69774)

ab69774 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam. We guarantee all our antibodies to still be viable if stored under the following conditions: 6 months at 4C 5 years at -20C 10 years at -80C I hope that this answers your question and if there ...

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