Anti-CD53 antibody [HI29] (CF405M) (ab115895)

Overview

  • Product name
    Anti-CD53 antibody [HI29] (CF405M)
    See all CD53 primary antibodies
  • Description
    Mouse monoclonal [HI29] to CD53 (CF405M)
  • Conjugation
    CF405M. Ex: 408nm, Em: 452nm
  • Specificity
    There is no cross-reactivity with erythrocytes or platelets. ab115895 recognizes a broad span of leucocytes of both normal and neoplastic plasma cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    PBMC (peripheral blood mononuclear cell) and Tonsil

  • Positive control
    • Human peripheral blood cells.
  • General notes

    CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.

Properties

Applications

Our Abpromise guarantee covers the use of ab115895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells. ab126026-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May be involved in growth regulation in hematopoietic cells.
  • Tissue specificity
    B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
  • Sequence similarities
    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI323659 antibody
    • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
    • CD 53 antibody
    • CD53 antibody
    • CD53 antigen antibody
    • CD53 antigen, isoform CRA_b antibody
    • CD53 glycoprotein antibody
    • Cd53 molecule antibody
    • CD53 tetraspan antigen antibody
    • CD53_HUMAN antibody
    • Cell surface glycoprotein CD53 antibody
    • Leukocyte antigen MRC OX-44 antibody
    • Leukocyte surface antigen CD53 antibody
    • MOX 44 antibody
    • MOX44 antibody
    • OTTHUMP00000059505 antibody
    • Ox-44 antibody
    • OX44 antibody
    • RATOX44 antibody
    • Tetraspanin 25 antibody
    • Tetraspanin-25 antibody
    • Tetraspanin25 antibody
    • Transmembrane glycoprotein antibody
    • TSPAN 25 antibody
    • Tspan-25 antibody
    • TSPAN25 antibody
    see all

Images

  • ab115895 at 5µl/106 cells staining CD53 in normal Human peripheral blood cells by Flow Cytometry (shown in yellow).
    Isotype control (blue).
    Cells in the Leukocyte gate were used for analysis (left).

References

ab115895 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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