Overview

  • Product nameAnti-CD53 antibody [MEM-53] (FITC)
    See all CD53 primary antibodies
  • Description
    Mouse monoclonal [MEM-53] to CD53 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Leukocytes of patient suffering from LGL-type leukemia.

  • Positive control
    • Human blood cells.
  • General notes

    The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

Applications

Our Abpromise guarantee covers the use of ab68565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. Use neat



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay be involved in growth regulation in hematopoietic cells.
  • Tissue specificityB-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI323659 antibody
    • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
    • CD 53 antibody
    • CD53 antibody
    • CD53 antigen antibody
    • CD53 antigen, isoform CRA_b antibody
    • CD53 glycoprotein antibody
    • Cd53 molecule antibody
    • CD53 tetraspan antigen antibody
    • CD53_HUMAN antibody
    • Cell surface glycoprotein CD53 antibody
    • Leukocyte antigen MRC OX-44 antibody
    • Leukocyte surface antigen CD53 antibody
    • MOX 44 antibody
    • MOX44 antibody
    • OTTHUMP00000059505 antibody
    • Ox-44 antibody
    • OX44 antibody
    • RATOX44 antibody
    • Tetraspanin 25 antibody
    • Tetraspanin-25 antibody
    • Tetraspanin25 antibody
    • Transmembrane glycoprotein antibody
    • TSPAN 25 antibody
    • Tspan-25 antibody
    • TSPAN25 antibody
    see all

Anti-CD53 antibody [MEM-53] (FITC) images

  • Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

References for Anti-CD53 antibody [MEM-53] (FITC) (ab68565)

This product has been referenced in:
  • Rasmussen AM  et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 153:4997-5007 (1994). Read more (PubMed: 7963560) »
  • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). Read more (PubMed: 2534480) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"