Overview

  • Product name
  • Description
    Chicken polyclonal to CD55
  • Specificity
    This antibody is specific for CD55.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: CGLPPDVPNA QPALEGRTSF PEDTVITYKC EESFVKIPGE KDSVICLKGS QWSDIEEFCN QKSCPNPGEI RNGQIDVPGG ILFGATISFS CNTG, corresponding to amino acids 36/129 of Human CD55

  • Positive control
    • E. coli-derived fusion protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 41 kDa.

Target

  • Function
    This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
  • Tissue specificity
    Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
  • Sequence similarities
    Belongs to the receptors of complement activation (RCA) family.
    Contains 4 Sushi (CCP/SCR) domains.
  • Domain
    The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
  • Post-translational
    modifications
    The Ser/Thr-rich domain is heavily O-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 55 antibody
    • CD55 antibody
    • CD55 antigen antibody
    • CD55 Cromer blood group system antibody
    • CD55 molecule (Cromer blood group) antibody
    • CD55 molecule antibody
    • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
    • Cd55a antibody
    • Complement decay accelerating factor antibody
    • Complement decay-accelerating factor antibody
    • Complement decay-accelerating factor, GPI-anchored antibody
    • CR antibody
    • CROM antibody
    • Cromer Blood Group antigen antibody
    • Cromer blood group system antibody
    • DAF antibody
    • Daf-GPI antibody
    • DAF_HUMAN antibody
    • Daf1 antibody
    • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
    • Decay accelarating factor 1, isoform CRA_a antibody
    • Decay accelerating factor (GPI-form) antibody
    • Decay Accelerating Factor for Complement antibody
    • Decay accelerating factor GPI-form antibody
    • Decay accelerating factor soluble-form antibody
    • GPI-DAF antibody
    • TC antibody
    see all

Anti-CD55 antibody images

  • Anti-CD55 antibody (ab37720) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg

    Secondary
    Goat anti-IgY-HRP. at 1/1000 dilution

    Predicted band size : 41 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-CD55 antibody (ab37720)

ab37720 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult Brain, 40 micron, floating)
Specification
Adult Brain, 40 micron, floating
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Joseph Dougherty

Verified customer

Submitted Aug 10 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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