Overview

  • Product nameAnti-CD55 antibody
    See all CD55 primary antibodies
  • Description
    Mouse monoclonal to CD55
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: DCGLPPDVPN AQPALEGRTS FPEDTVITYK CEESFVKIPG EKDSVICLKG SQWSDIEEFC NRSCEVPTRL NSASLKQPYI TQNYFPVGTV VEYECRPGYR , corresponding to amino acids 35-135 of Human CD55

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Lung.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
    • Tissue specificityExpressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
    • Sequence similaritiesBelongs to the receptors of complement activation (RCA) family.
      Contains 4 Sushi (CCP/SCR) domains.
    • DomainThe first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
    • Post-translational
      modifications
      The Ser/Thr-rich domain is heavily O-glycosylated.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 55 antibody
      • CD55 antibody
      • CD55 antigen antibody
      • CD55 Cromer blood group system antibody
      • CD55 molecule antibody
      • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
      • Cd55a antibody
      • Complement decay accelerating factor antibody
      • Complement decay-accelerating factor antibody
      • Complement decay-accelerating factor, GPI-anchored antibody
      • CR antibody
      • CROM antibody
      • Cromer Blood Group antigen antibody
      • Cromer blood group system antibody
      • DAF antibody
      • Daf-GPI antibody
      • DAF_HUMAN antibody
      • Daf1 antibody
      • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
      • Decay accelarating factor 1, isoform CRA_a antibody
      • Decay accelerating factor (GPI-form) antibody
      • Decay Accelerating Factor for Complement antibody
      • Decay accelerating factor GPI-form antibody
      • Decay accelerating factor soluble-form antibody
      • GPI-DAF antibody
      • TC antibody
      see all

    Anti-CD55 antibody images

    • IHC image of CD55 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54595, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



    • Predicted band size : 41 kDa
      CD55 antibody (ab54595) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-CD55 antibody (ab54595)

    ab54595 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 30 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 20 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"