Overview

  • Product nameAnti-CD57 antibody [NK-1] (PE/Cy5®)
    See all CD57 primary antibodies
  • Description
    Mouse monoclonal [NK-1] to CD57 (PE/Cy5®)
  • ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, 0.2% Gelatin, PBS; Stabilizing agent
  • Concentration information loading...
  • PurityPurified IgM
  • ClonalityMonoclonal
  • Clone numberNK-1
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • FunctionInvolved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
  • Tissue specificityMainly expressed in the brain.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyltransferase 43 family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-glucuronyltransferase 1 antibody
    • 3-glucuronyltransferase antibody
    • B3GA1_HUMAN antibody
    • B3gat1 antibody
    • Beta 1 3 glucuronyltransferase 1 antibody
    • Beta-1 antibody
    • Beta-1,3-glucuronyltransferase 1 antibody
    • CD 57 antibody
    • CD57 antibody
    • CD57 antigen antibody
    • Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody
    • Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody
    • GlcAT P antibody
    • GlcAT-P antibody
    • GLCATP antibody
    • GlcUAT P antibody
    • GlcUAT-P antibody
    • GlcUATP antibody
    • Glucuronosyltransferase P antibody
    • HNK 1 antibody
    • HNK1 antibody
    • LEU 7 antibody
    • LEU7 antibody
    • LEU7 antigen antibody
    • NK 1 antibody
    • NK1 antibody
    • UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody
    • UDP-GlcUA:glycoprotein beta-1 antibody
    see all

References for Anti-CD57 antibody [NK-1] (PE/Cy5®) (ab25445)

This product has been referenced in:
  • Jilek S  et al. HLA-B7-restricted EBV-specific CD8+ T cells are dysregulated in multiple sclerosis. J Immunol 188:4671-80 (2012). Human . Read more (PubMed: 22461701) »
  • Lopez-Vergès S  et al. Expansion of a unique CD57?NKG2Chi natural killer cell subset during acute human cytomegalovirus infection. Proc Natl Acad Sci U S A 108:14725-32 (2011). IF ; Human . Read more (PubMed: 21825173) »

See all 3 Publications for this product

Product Wall

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Spectral Red is a PE/CY5 tandem conjugate. They are the same fluorophore but have different names.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

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I have contacted our lab who have suggested IgM, [11E10] (Spectral Red) - Isotype Control as a suitable isotype control for ab25445. We have now added this item to our catalogue, reference number ab151937 (http:/...

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I am very sorry that we do not stock this product and understand your frustration. I will contact the lab to see whether we could provide this isotype control and if so if it can be added to the catalogue. I will...

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Thank you for your inquiry.

Unfortunately, we do not have any isotype control mouse IgM PE-Cy5 conjugated available in our catalog.
I would like to recommend checking the Biocompare website which has an excellent antibody search facilit...

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Thanks for your reply. My colleague is out of the office today but I'll be happy to assist you untl she returns.

I expect that it will take 1-2 weeks to get this antibody to you. I'm currently working on getting a new lot, and we will send it...

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Thank you for contacting us.


The lab has informed me that ab25445is designed so that very minimal (typically <3%) FL2/FL3 compensation is required to remove any bleed in the PE channel. I hope this helps, please let me know if you need a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"