Anti-CD57 antibody [NK-1] (Phycoerythrin) (ab25577)

Overview

  • Product nameAnti-CD57 antibody [NK-1] (Phycoerythrin)
    See all CD57 primary antibodies
  • Description
    Mouse monoclonal [NK-1] to CD57 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.2% Sodium azide
    Constituents: PBS, 16% Sucrose
    Note: Also contains a stabilizing agent.
  • Concentration information loading...
  • PurityPurified IgM
  • ClonalityMonoclonal
  • Clone numberNK-1
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab154448-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionInvolved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
  • Tissue specificityMainly expressed in the brain.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyltransferase 43 family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-glucuronyltransferase 1 antibody
    • 3-glucuronyltransferase antibody
    • B3GA1_HUMAN antibody
    • B3gat1 antibody
    • Beta 1 3 glucuronyltransferase 1 antibody
    • Beta-1 antibody
    • Beta-1,3-glucuronyltransferase 1 antibody
    • CD 57 antibody
    • CD57 antibody
    • CD57 antigen antibody
    • Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody
    • Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody
    • GlcAT P antibody
    • GlcAT-P antibody
    • GLCATP antibody
    • GlcUAT P antibody
    • GlcUAT-P antibody
    • GlcUATP antibody
    • Glucuronosyltransferase P antibody
    • HNK 1 antibody
    • HNK1 antibody
    • LEU 7 antibody
    • LEU7 antibody
    • LEU7 antigen antibody
    • NK 1 antibody
    • NK1 antibody
    • UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody
    • UDP-GlcUA:glycoprotein beta-1 antibody
    see all

References for Anti-CD57 antibody [NK-1] (Phycoerythrin) (ab25577)

This product has been referenced in:
  • Mondal AM  et al. p53 isoforms regulate aging- and tumor-associated replicative senescence in T lymphocytes. J Clin Invest 123:5247-57 (2013). Flow Cyt ; Human . Read more (PubMed: 24231352) »
  • Abo T & Balch CM A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol 127:1024-9 (1981). Read more (PubMed: 6790607) »

See all 2 Publications for this product

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All Abcam products are for research use only and not intended for diagnostic or therapeutic use.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more...

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Thank you for your enquiry. Unfortunately the light chain of anti-CD57 PE (ab25577) has not been classified as heavy or light chain. We DO have several IgM isotype controls; ab18400 Mouse IgM, kappa (MM-30) - isotype control ab18401 Mouse IgM,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"