Overview

  • Product nameAnti-CD59 antibody [MEM-43]
    See all CD59 primary antibodies
  • Description
    Mouse monoclonal [MEM-43] to CD59
  • SpecificityCD59 antigen (human). MEM-43 identified CD59 as the new cluster on 4th HLDA Workshop. MEM-43 reacts with well defined epitope (WhO, P-53).
  • Tested applicationsSuitable for: IHC-Fr, WB, IP, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Thymocytes and T lymphocytes.

  • Positive control
    • Placenta
  • General notes

    When originally tested in WB, SDS was included in the sample buffer, however, feedback from one researcher has shown that it is best to omit SDS from the sample buffer.

Properties

Applications

Our Abpromise guarantee covers the use of ab9182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non-reducing conditions.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 - 2 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 17911601
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionPotent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
    The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
  • Involvement in diseaseDefects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
  • Sequence similaritiesContains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications
    N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
    Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
  • Cellular localizationCell membrane. Secreted. Soluble form found in a number of tissues.
  • Information by UniProt
  • Database links
  • Alternative names
    • 16.3A5 antibody
    • 1F5 antibody
    • 1F5 antigen antibody
    • 20 kDa homologous restriction factor antibody
    • CD 59 antibody
    • CD_antigen=CD59 antibody
    • CD59 antibody
    • CD59 antigen antibody
    • CD59 antigen complement regulatory protein antibody
    • CD59 antigen p18 20 antibody
    • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
    • CD59 glycoprotein antibody
    • CD59 molecule antibody
    • CD59 molecule complement regulatory protein antibody
    • CD59_HUMAN antibody
    • Cd59a antibody
    • Complement regulatory protein antibody
    • EJ16 antibody
    • EJ30 antibody
    • EL32 antibody
    • FLJ38134 antibody
    • FLJ92039 antibody
    • G344 antibody
    • HRF 20 antibody
    • HRF-20 antibody
    • HRF20 antibody
    • Human leukocyte antigen MIC11 antibody
    • Ly 6 like protein antibody
    • Lymphocytic antigen CD59/MEM43 antibody
    • MAC inhibitory protein antibody
    • MAC IP antibody
    • MAC-inhibitory protein antibody
    • MAC-IP antibody
    • MACIF antibody
    • MACIP antibody
    • MEM43 antibody
    • MEM43 antigen antibody
    • Membrane attack complex (MAC) inhibition factor antibody
    • Membrane attack complex inhibition factor antibody
    • Membrane inhibitor of reactive lysis antibody
    • MGC2354 antibody
    • MIC11 antibody
    • MIN1 antibody
    • MIN2 antibody
    • MIN3 antibody
    • MIRL antibody
    • MSK21 antibody
    • p18 20 antibody
    • Protectin antibody
    • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
    • T cell activating protein antibody
    see all

Anti-CD59 antibody [MEM-43] images

  • ab9182 staining CD59 in Human fibrosarcoma (HT1080) by Flow Cytometry. Cells were fixed with paraformaldehyde. The  sample was incubated with the primary antibody,10 µg/ml in PBS, for 1 hour at 20°C. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody.

    See Abreview

References for Anti-CD59 antibody [MEM-43] (ab9182)

This product has been referenced in:
  • Dun MD  et al. The Chaperonin Containing TCP1 Complex (CCT/TRiC) Is Involved in Mediating Sperm-Oocyte Interaction. J Biol Chem 286:36875-87 (2011). WB, ICC/IF ; Mouse . Read more (PubMed: 21880732) »
  • Kolev MV  et al. Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration. Pharmacogenomics J 10:12-9 (2010). Flow Cyt ; Human . Read more (PubMed: 19884909) »

See all 10 Publications for this product

Product Wall

Thank you for contacting us. I am sorry to hear that you are having trouble using this antibody.

I have read the information you have kindly provided and would like to ask few questions for better understanding of the problem;

- Co...

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We have removed functional studies from the datasheet for ab9182 since the sodium azide present in the storage buffer is toxic to cells.

Alternatively, we have a sodium azide free anti-CD59 antibody that has been tested in functional stud...

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Thank you for contacting us.
For ab9182 the ab97244 is indeed a compatible secondary antibody that can be used for ICC/IF.
The ab98794 is an IgG antibody which can cross react with IgG2a however because we do not have tested data so we can't ...

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According to our records, ab9182 was proving difficult to use inIHC and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand ...

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Thank you for calling Abcam.

I am sorry that you were having problems with ab9182 in IHC-P. As we discussed, I am sending you ab9183 as a free of charge replacement and you should have it by 10.30am tomorrow morning. You r new order number is...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Fibrosarcoma (HT1080))
Specification Fibrosarcoma (HT1080)
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy no gating
Username

Abcam user community

Verified customer

Submitted Aug 13 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Aug 19 2008

These two monoclonals (ab9182, ab9183) have not been tested for cross reactivity with mouse CD59.

Many thanks for this useful information which we will certainly make available via the online datasheet for this product. I note the originator of this antibody advised that it works under conditions of SDS-PAGE, but the sample buffer should not contai...

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It works under conditions of SDS-PAGE, but the sample buffer should not contain reducing agents (DTT, Mercaptoethanol). SDS presence in the buffer is fine.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"