Anti-CD6/T12 antibody (ab231314)
Key features and details
- Rabbit polyclonal to CD6/T12
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD6/T12 antibody
See all CD6/T12 primary antibodies -
Description
Rabbit polyclonal to CD6/T12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse CD6/T12 aa 1-250. Expressed in E.coli. N-terminal tags.
Database link: Q61003 -
Positive control
- IHC-P: Mouse spleen tissue. WB: Jurkat whole cell lysate (ab7899); Mouse lymph node lysate; Recombinant mouse CD6/T12 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231314 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 72 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 72 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Involved in cell adhesion. Binds to CD166. -
Tissue specificity
Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. -
Sequence similarities
Contains 3 SRCR domains. -
Post-translational
modificationsAfter T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Contains intrachain disulfide bond(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 923 Human
- Entrez Gene: 12511 Mouse
- Omim: 186720 Human
- SwissProt: P30203 Human
- SwissProt: Q61003 Mouse
- Unigene: 290897 Mouse
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Alternative names
- CD_antigen=CD6 antibody
- CD6 antibody
- CD6 antigen Tp120 antibody
see all
Images
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Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD6/T12 using ab231314 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-CD6/T12 antibody (ab231314) at 2 µg/ml + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 72 kDa -
Anti-CD6/T12 antibody (ab231314) at 2 µg/ml + Mouse lymph node lysate
Predicted band size: 72 kDa -
Anti-CD6/T12 antibody (ab231314) at 2 µg/ml + Recombinant mouse CD6/T12 protein
Predicted band size: 72 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231314 has not yet been referenced specifically in any publications.