Recombinant
RabMAb

Anti-CD6 antibody [EPR4056] (ab109218)

Overview

  • Product name
    Anti-CD6 antibody [EPR4056]
    See all CD6 primary antibodies
  • Description
    Rabbit monoclonal [EPR4056] to CD6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human CD6.

  • Positive control
    • WB: Jurkat, HuT-78, PBMC and Human tonsil lysates IHC-P: Human tonsil and Human lymphocytes infiltrating colon tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 72 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Involved in cell adhesion. Binds to CD166.
    • Tissue specificity
      Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
    • Sequence similarities
      Contains 3 SRCR domains.
    • Post-translational
      modifications
      After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
      Contains intrachain disulfide bond(s).
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD_antigen=CD6 antibody
      • CD6 antibody
      • CD6 antigen Tp120 antibody
      • CD6 molecule antibody
      • CD6_HUMAN antibody
      • FLJ44171 antibody
      • OX52 antibody
      • T cell differentiation antigen CD6 antibody
      • T-cell differentiation antigen CD6 antibody
      • T12 antibody
      • TP120 antibody
      see all

    Images

    • All lanes : Anti-CD6 antibody [EPR4056] (ab109218) at 1/1000 dilution

      Lane 1 : Jurkat lysates
      Lane 2 : HuT-78 lysates
      Lane 3 : PBMC lysates
      Lane 4 : Human tonsil lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 72 kDa
      Observed band size: 90-130 kDa (why is the actual band size different from the predicted?)

    • ab109218, at a 1/100 dilution, staining CD6 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • ab109218, at a 1/100 dilution, staining CD6 in paraffin embedded Human lymphocytes infiltrating colon tissue by Immunohistochemistry.
    • ab109218 showing negative staining in Normal kidney tissue.

    • ab109218 showing negative staining in Normal uterus tissue.

    • ab109218 showing negative staining in Normal brain tissue.

    References

    ab109218 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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