Anti-CD6 antibody [MEM-98] (Phycoerythrin) (ab59053)

Overview

  • Product nameAnti-CD6 antibody [MEM-98] (Phycoerythrin)
    See all CD6 primary antibodies
  • Description
    Mouse monoclonal [MEM-98] to CD6 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThe antibody reacts with CD6, a 100-130 kD single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.
  • Tested applicationsSuitable for: IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human CD6 antigen purified by immunoaffinity chromatography from HBP ALL cells followed by preparative SDS-PAGE of non boiled non reduced sample (excised piece of gel corresponding to the 100 kDa zone).

  • General notesDo not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

    The purified antibody was conjugated with R Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Properties

Applications

Our Abpromise guarantee covers the use of ab59053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
Flow Cyt
  • Application notesFlow Cyt: Use 20µl for 106 cells or 100µl whole blood.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Use under non reducing conditions. Predicted molecular weight: 100 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in cell adhesion. Binds to CD166.
    • Tissue specificityExpressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
    • Sequence similaritiesContains 3 SRCR domains.
    • Post-translational
      modifications
      After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
      Contains intrachain disulfide bond(s).
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD_antigen=CD6 antibody
      • CD6 antibody
      • CD6 antigen Tp120 antibody
      • CD6 molecule antibody
      • CD6_HUMAN antibody
      • FLJ44171 antibody
      • OX52 antibody
      • T cell differentiation antigen CD6 antibody
      • T-cell differentiation antigen CD6 antibody
      • T12 antibody
      • TP120 antibody
      see all

    References for Anti-CD6 antibody [MEM-98] (Phycoerythrin) (ab59053)

    ab59053 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab59053.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"