Overview

  • Product nameAnti-CD62E antibody [1.2B6]See all CD62E primary antibodies ...
  • Description
    Mouse monoclonal [1.2B6] to CD62E
  • SpecificityThis antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.
  • Tested applicationsELISA, Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Human E-Selectin (ELAM-1)

  • General notes


    This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab6630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Flow Cyt Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Non-reduced only.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • Sequence similaritiesBelongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 62E antibody
      • CD26E antibody
      • CD62 antigen-like family member E antibody
      • CD62E antibody
      • CD62E antigen antibody
      • E selectin antibody
      • E selectin antibody
      • E-selectin antibody
      • ELAM 1 antibody
      • ELAM antibody
      • ELAM-1 antibody
      • ELAM1 antibody
      • Endothelial adhesion molecule 1 antibody
      • Endothelial leukocyte adhesion molecule 1 antibody
      • ESEL antibody
      • LECAM 2 antibody
      • LECAM2 antibody
      • Leukocyte endothelial cell adhesion molecule 2 antibody
      • Leukocyte-endothelial cell adhesion molecule 2 antibody
      • LYAM2_HUMAN antibody
      • SELE antibody
      • Selectin E antibody
      • SelectinE antibody
      see all

    Anti-CD62E antibody [1.2B6] images

    • ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

    References for Anti-CD62E antibody [1.2B6] (ab6630)

    This product has been referenced in:
    • Zhou H  et al. NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion. J Pharmacol Exp Ther 334:260-8 (2010). WB ; Human . Read more (PubMed: 20378716) »
    • Akinci MA  et al. MOLECULAR PROFILING OF THE OF CONJUNCTIVAL EPITHELIAL STEM SIDE POPULATION CELLS IDENTIFIES ATYPICAL CELL SURFACE MARKERS AND SOURCES OF A SLOW CYCLING PHENOTYPE. Invest Ophthalmol Vis Sci : (2009). Read more (PubMed: 19324848) »

    See all 6 Publications for this product

    Product Wall

    Thank you for contacting us.

    Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

    However, instead of using PFA as a fixative, I w...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (human nasal tissue)
    Loading amount 90 µg
    Specification human nasal tissue
    Gel Running Conditions Non-reduced Denaturing (10 %)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Michael Könnecke

    Verified customer

    Submitted Jan 11 2013

    Thank you for contacting us.

    You are quite correct. As CD62E is a single pass membrane bound protein, there should be no need to permeabilze the cells for an immunocytochemical stain.

    I hope this information is helpful to you....

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Human Cell (HUVEC)
    Specification HUVEC
    Type Direct
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 07 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Intestine)
    Specification Intestine
    Fixative Paraformaldehyde
    Permeabilization Yes - Citrate Buffer
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2007

    I have received feedback from the source of ab6630 and ab19264 and would like to share their feedback. Unfortunately I am still awaiting feedback regarding ab2213. For ab6630 we would suggest a working dilution of 10ug/ml. For ab19264 we recommend...

    Read More

    The antibody will recognise the native form of the epitope thus Westerns must be conducted in non-reducing conditions (i.e. without DTT/ beta-mercaptoethanol

    The antibody has been characterised extensively and published in: J Immunol 1990 Apr 1;144(7):2558-65 A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. Well...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"