Overview

  • Product name
    Anti-CD62E antibody [1.2B6]
    See all CD62E primary antibodies
  • Description
    Mouse monoclonal [1.2B6] to CD62E
  • Specificity
    This antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, IHC-Fr, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Human E-Selectin (ELAM-1)

  • General notes


    This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab6630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Non-reduced only.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • Sequence similarities
      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD26E antibody
      • CD62 antigen-like family member E antibody
      • CD62E antibody
      • E selectin antibody
      • E-selectin antibody
      • ELAM antibody
      • ELAM-1 antibody
      • ELAM1 antibody
      • Endothelial adhesion molecule 1 antibody
      • Endothelial leukocyte adhesion molecule 1 antibody
      • ESEL antibody
      • LECAM2 antibody
      • Leukocyte-endothelial cell adhesion molecule 2 antibody
      • LYAM2_HUMAN antibody
      • Sele antibody
      • Selectin E antibody
      see all

    Images

    • ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

    References

    This product has been referenced in:
    • Allen CL  et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). Read more (PubMed: 24205233) »
    • Zhou H  et al. NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion. J Pharmacol Exp Ther 334:260-8 (2010). WB ; Human . Read more (PubMed: 20378716) »

    See all 8 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (human nasal tissue)
    Loading amount
    90 µg
    Specification
    human nasal tissue
    Gel Running Conditions
    Non-reduced Denaturing (10 %)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Michael Könnecke

    Verified customer

    Submitted Jan 11 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Cell (HUVEC)
    Specification
    HUVEC
    Type
    Direct
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 07 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Intestine)
    Specification
    Intestine
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Citrate Buffer
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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