Overview

  • Product name
    Anti-CD62E antibody [1.2B6]
    See all CD62E primary antibodies
  • Description
    Mouse monoclonal [1.2B6] to CD62E
  • Specificity
    This antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, IHC-Fr, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Human E-Selectin (ELAM-1)

  • General notes


    This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab6630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Non-reduced only.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • Sequence similarities
      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD26E antibody
      • CD62 antigen-like family member E antibody
      • CD62E antibody
      • E selectin antibody
      • E-selectin antibody
      • ELAM antibody
      • ELAM-1 antibody
      • ELAM1 antibody
      • Endothelial adhesion molecule 1 antibody
      • Endothelial leukocyte adhesion molecule 1 antibody
      • ESEL antibody
      • LECAM2 antibody
      • Leukocyte-endothelial cell adhesion molecule 2 antibody
      • LYAM2_HUMAN antibody
      • Sele antibody
      • Selectin E antibody
      see all

    Images

    • ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

    References

    This product has been referenced in:
    • Allen CL  et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). Read more (PubMed: 24205233) »
    • Zhou H  et al. NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion. J Pharmacol Exp Ther 334:260-8 (2010). WB ; Human . Read more (PubMed: 20378716) »

    See all 8 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

    However, instead of using PFA as a fixative, I w...

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    Thank you for contacting us.

    You are quite correct. As CD62E is a single pass membrane bound protein, there should be no need to permeabilze the cells for an immunocytochemical stain.

    I hope this information is helpful to you....

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    I have received feedback from the source of ab6630 and ab19264 and would like to share their feedback. Unfortunately I am still awaiting feedback regarding ab2213. For ab6630 we would suggest a working dilution of 10ug/ml. For ab19264 we recommend...

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    The antibody will recognise the native form of the epitope thus Westerns must be conducted in non-reducing conditions (i.e. without DTT/ beta-mercaptoethanol

    The antibody has been characterised extensively and published in: J Immunol 1990 Apr 1;144(7):2558-65 A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. Well...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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