Human CD62E ELISA Set (without plates) (ab47348)
Overview
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Product name
Human CD62E ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.5 ng/ml -
Range
1 ng/ml - 32 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CD26E Human in vitro ELISA Set is designed for the quantitative measurement of CD26E in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The CD62E ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA set is intended to measure human CD62E in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant human CD62E.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 10 x 96 tests 5 x 96 tests CD62E Capture Antibody 2 x 0.5ml 1 x 0.5ml CD62E standard Yellow 10 vials 5 vials Chromogen TMB Substrate Solution 4 vials 2 vials Detection biotinylated anti-CD62E antibody 2 x 0.55ml 1 x 0.55ml Streptavidin-HRP 2 vials 1 vial -
Research areas
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Relevance
CD62E is expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by CD62E is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids). -
Cellular localization
Membrane; single-pass type I membrane protein -
Alternative names
- ELAM
- ELAM1
- ESEL
see all -
Database links
- Entrez Gene: 6401 Human
- SwissProt: P16581 Human
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab47348 has not yet been referenced specifically in any publications.