CD62E (Selectin) Human ELISA Kit (ab100512)

Overview

  • Product nameCD62E (Selectin) Human ELISA KitSee all CD62E kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.39 83% - 103%
    Serum 93.75 83% - 102%
    Plasma 95.38 84% - 105%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD62E (Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62E in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD62E coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • Sequence similaritiesBelongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P16581 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • CD62 antigen-like family member E
    • CD62E
    • CD62E antigen
    • E selectin
    • E-selectin
    • ELAM
    • ELAM-1
    • ELAM1
    • Endothelial leukocyte adhesion molecule 1
    • ESEL
    • LECAM2
    • Leukocyte endothelial cell adhesion molecule 2
    • Leukocyte-endothelial cell adhesion molecule 2
    • LYAM2_HUMAN
    • Sele
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

CD62E (Selectin) Human ELISA Kit images

  • Representative Standard Curve using ab100512.

  • Representative Standard Curve using ab100512.

Protocols

References for CD62E (Selectin) Human ELISA Kit (ab100512)

ab100512 has not yet been referenced specifically in any publications.

Product Wall

Yes, I can confirm that EDTA, Heparin, or Citrate collected plasma can be used with the ab100512 CD62E Human ELISA Kit

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

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Thank you for your inquiry and I apologize for the delay while I was awaiting more information from our testing laboratory. There is a way that you can to test for TMB and HRP-streptavidin activity: Prepare a 100-fold dilution of HRP-Streptavidin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"