Overview

  • Product nameAnti-CD62L antibody [1H3] (FITC)
    See all CD62L primary antibodies
  • Description
    Mouse monoclonal [1H3] to CD62L (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab52068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. in 100 µl PBS, or for 100µl of blood.



ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies Use at an assay dependent concentration. Can be used for monitoring of T cells in peripheral blood; study of Cell-adhesion molecules; study of B cell functions; study of lymphomas and leukemias.

Target

  • FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • Tissue specificityExpressed in B cell lines and T lymphocytes.
  • Sequence similaritiesBelongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Anti-CD62L antibody [1H3] (FITC) images

  • ab52068 staining human granulocytes in whole blood by Flow Cytometery. Cells were treated with Optilyse C (containing 1.5 % paraformaldehyde) solution and gated by electronic volume and side scatter. The primary antibody was diluted 1/10 and incubated with sample for 30 minutes at 21°C.

    See Abreview

References for Anti-CD62L antibody [1H3] (FITC) (ab52068)

This product has been referenced in:
  • Barlow AK  et al. Exogenously provided peptides of a self-antigen can be processed into forms that are recognized by self-T cells. J Exp Med 187:1403-15 (1998). Read more (PubMed: 9565633) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Granulocytes in whole blood)
Specification Granulocytes in whole blood
Preparation Cell harvesting/tissue preparation method: whole blood (EDTA) treated with Optilyse C (Beckman Coulter) to lyse erythrocytes and fic leukocytes
Sample buffer: Diluted in PBS
Fixation Optilyse C (contains 1.5 % paraformaldehyde)
Permeabilization No
Gating Strategy Granulocytes gated by electonic volume and side scatter
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Submitted Nov 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"