The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells. in 100 µl PBS, or for 100µl of blood.
ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. Can be used for monitoring of T cells in peripheral blood; study of Cell-adhesion molecules; study of B cell functions; study of lymphomas and leukemias.
FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Tissue specificityExpressed in B cell lines and T lymphocytes.
Sequence similaritiesBelongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.
Flow Cytometry - CD62L antibody [1H3] (FITC) (ab52068)This image is courtesy of an anonymous Abreview
ab52068 staining human granulocytes in whole blood by Flow Cytometery. Cells were treated with Optilyse C (containing 1.5 % paraformaldehyde) solution and gated by electronic volume and side scatter. The primary antibody was diluted 1/10 and incubated with sample for 30 minutes at 21°C.